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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene continues to be observed in many tumors, which leads to chromosome instability, and therefore may perhaps contribute to tumorigenesis. This protein was discovered to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a significant function in marketing cell-cycle development in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have been described. Two extra splice variants ended up noted but in depth nucleotide sequence details is not really yet readily available.
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Blocking peptides are peptides that bind exclusively on the target antibody and block antibody binding. These peptide normally contains the epitope identified by the antibody.
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This guidebook outlines further achievable optimization of mobile and immunoassay parameters to be sure the best possible effects are...
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This guide outlines even further attainable optimization of cellular and immunoassay parameters to be certain the absolute best effects are...
Antibodies bound to the blocking peptide not bind towards the epitope on the focus on protein. By comparing the staining with the blocked antibody versus the antibody on your own, you can see which staining is certain.
Focus on Information Cyclin E belongs for the remarkably conserved cyclin spouse and children, whose members are characterized by a spectacular periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which lead on the temporal coordination of every mitotic occasion. Cyclin E types a fancy with and functions as a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S transition. This protein accumulates in the G1-S phase boundary and it is degraded as cells progress through S phase.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...
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